Anti-KLH; clone 3B1

Application

Research CategoryInflammation & Immunology

Anti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Substances with low molecular weights are generally not immunogenic and require the aid of carrier proteins to stimulate an immune response. Carrier proteins have relatively high molecular weights and carry many epitopes. An immune response to a hapten-carrier conjugate results in antibodies that are specific for both the hapten and the carrier. Hemocyanin is a high molecular weight respiratory metalloprotein found in the hemolymph of many mollusks and crustaceans. Molluscan hemocyanin is based on an approximately 400 kDa polypeptide subunit that is folded into a chain of multiple, immunologically distinct domains, each with a binuclear copper site. Keyhole Limpet Hemocyanin (KLH), the hemocyanin from the giant keyhole limpet, Megathura crenulata, is the most widely used carrier protein. It is an extremely large protein (6 - 7.5 million daltons) that can exist as a di-decamer, multi-decamers, or flexible tubules of varying length. It is easily dissociated into subunits and the dissociation-association reaction depends on protein concentration, ionic strength, and pH. At pH 6.5, KLH exists in a fully associated state without any evidence of dissociation products. In contrast, at pH 8.5 in borate saline the molecule is completely dissociated.

Immunogen

Purified Keyhole limpet hemocyanin from Megathura crenulata

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified by ion-exchange chromatography

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting with Keyhole Limpet Hemocyanin (KLH).

Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.

Specificity

Clone 3B1 detects keyhole limpet hemocyannin (KLH) and KLH conjugated to peptide or protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~375 kDa observed; Calculated ~ 6 to 7.5 MDa (undissociated). Uncharacterized bands may be observed in some lysate(s).